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KMID : 0928520010110040460
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Korean Journal of Lipidology
2001 Volume.11 No. 4 p.460 ~ p.467
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Peroxisome Proliferator Activated ReceptorγGene Expression in Cultured Human Skeletal Muscle Cell - Effect of Insulin and Fatty Acid -
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Kim Jeong-Mi
Lim Soo
Kim Tae-Yong
Cho Young-Min
Shin Won-Sik
Hong Hye-Kyung
Park Kyong-Soo
Kim Seong-Yeon
Lee Hong-Kyu
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Abstract
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Objective: The peroxisome proliferator activated receptor gamma (PPARγ) has been identified as a nuclear receptor for thiazolidinediones which show insulin sensitizing properties. PPARγis present in skeletal muscle -major site of insulin resistance as well as adipose tissue. PPARγexpression is increased in skeletal muscle of obese and obese type 2 diabetic patients and positively correlated with body mass index and fasting plasma insulin. However, regulation of PPARγexpression in skeletal muscle is largely unknown. To determine the effect of hyperinsulinemia and increased availability of free fatty acid on PPARγgene expression, we quantified PPARγmRNA abundance and protein expression of cultured human skeletal muscle cells from non-diabetic subjects.
Materials and Methods: Cultured human skeletal muscle cells from non-diabetic subjects were fused and differentiated for 4 days. During fusion, insulin (final concentration 30 M) or 10 % intralipid (final concentration 100 mg/dL) was added every 2 days. At the end of fusion and differentiation, cells were harvested. PPARγmRNA abundance and protein expression were quantified using competitive reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis.
Results: Compared with untreated muscle cells, those treated with 10% intralipid showed significantly increased PPARγmRNA and protein expression (p < 0.05 respectively). Muscle cells treated with high insulin concentration did not show changes in PPARγ expression.
Conclusion: PPARγgene expression in cultured human skeletal muscle cell is upregulated by chronically increased fatty acid availability but not by chronically elevated insulin concentration.
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KEYWORD
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Human skeletal muscle, PPARγ, Insulin resistance, Fatty acid
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